Akira is the roommate of Mai Tokiha's younger brother Takumi Tokiha and attends the Fuka Middle school with him. Akira's strong-willed and forceful personality contrasts with Takumi's kind and (relatively) feminine attitude and is extremely private, setting up a curtain and strict boundaries for the room. Akira is also a student of Ishigami, the art teacher.

Edward Wong Hau Pepelu Tivrusky IV is also known as Radical Edward and Ed. Her birth name is Françoise Appledehli (born January 1, 2058). She is a hacker prodigy from Earth. She is a comically eccentric teenage girl around 13 years in age.

Her somewhat androgynous appearance often causes her to be mistaken for a boy as in her first appearance, Faye thought she was a boy because she looked similar to a boy, which also implies that she hasn't hit puberty yet.

Kino is the main protagonist in the series and travels to different countries with her talking motorcycle Hermes, discovering their cultures and people. In the anime, Kino's gender is ambiguous in the beginning, but is later confirmed to be female in the fourth episode, in which she first meets Hermes and borrows the name Kino from another traveler. She is skilled in combat, carrying both guns and knives, and is accustomed to life as a traveler. To those she meets, she is invariably polite and answers questions directly. Her gender is not initially apparent to the viewer, because she is almost flat chested (despite being in her mid-teens), heavily armed, and dresses in baggy masculine motorcycle gear, as well as her usage of a masculine pronoun to refer to herself.