Second year in Kasensou Private Prepatory Academy. Mayuri's classmate. Has the personality of a polite, well-groomed and justified kind of beautiful girl (though a guy). Uses honorifics specifically with Rintarou. Doesn't push his opinions much upon others, but when he does he's quite bashful and blushes quite a bit. Because he's quite serious by nature, he simply believes what others say.

Kino is the main protagonist in the series and travels to different countries with her talking motorcycle Hermes, discovering their cultures and people. In the anime, Kino's gender is ambiguous in the beginning, but is later confirmed to be female in the fourth episode, in which she first meets Hermes and borrows the name Kino from another traveler. She is skilled in combat, carrying both guns and knives, and is accustomed to life as a traveler. To those she meets, she is invariably polite and answers questions directly. Her gender is not initially apparent to the viewer, because she is almost flat chested (despite being in her mid-teens), heavily armed, and dresses in baggy masculine motorcycle gear, as well as her usage of a masculine pronoun to refer to herself.