This story's main character. The game operates along her point of view. She is one person of the Anti-Demon Ninja. She is a female ninja of justice going against the demon people's heretical doctrine.

Holo is a wolf harvest deity originally from a land in the north known as Yoitsu. She made a promise with men from a town called Pasroe where she would ensure the town would have good wheat harvests year after year. However, as time went on, the townspeople slowly started to forsake Holo and did not rely on her as they once did. As such, Holo escapes from town in Lawrence's wagon and started traveling with him to see how much the world has changed since she has been in Pasroe. Holo has chosen the form of a girl younger than Lawrence, though she still retains her large white-tipped wolf tail, and ears. Her true form is that of a very large wolf which many people revered and feared.

Aya is the younger sister of Shin Natsume, and Maya Natsume. She is a freshman at Toudou Academy. She has a similar build to her sister and is close to equaling her in bust size. Since the first time they met, she has decided to become Souichiro Nagi’s wife because she believes it's fate; and it follows the tradition of the Natsume women. This attraction to Souichiro also leads to a rift between her and her sister, as Souichiro is in love with Maya. Though out the series, she has increased her skill and dedication so she can support Souichirou in anyway.

Yoruichi Shihouin is an attractive, dark-skinned woman who can transform into a black cat for long periods of time. She is intelligent and witty, and is intimately knowledgeable of Soul Society and its workings, as she was the former leader of the special forces. Although of noble birth, she acts differently from other nobles. For instance, she instructed Soifon to refer to her without using honorific suffixes. She also transforms into her human form directly in front of Ichigo on several occasions just to see his reaction to seeing her naked.

First mistaken by Maka and Soul to be a witch that they needed to make Soul a death scythe, it turns out that she is just a cat with strong magical powers. The theme of Blair’s magic consists of pumpkins, which she uses as weapons (bombs, cannon, etc). She has started to like Soul (who she calls Scythe Boy) and playfully flirts with him(it is unknown if this is playfulness or real feelings). Blair also flirts with Soul to see how Maka acts by doing so. She was suppose to be the witch soul Maka and Soul needed but since she wasn’t a witch, they took one of her nine “lives”. Taking one of her lives meant that they had to start all over again. She is well endowed, which is used when she flirts with Soul usually by appearing out of her cat form in little to no clothing. She is very good at understanding other people. She is always looking for some fun, whether it is harmless or not. Her hat is named Zwan and can change into a hand when Blair commands it to. She sometimes refers to herself in third person.

The female protagonist of To Love-Ru, Lala is the princess of Deviluke. A rather childish girl, she is somewhat naive of the culture and familiarities on Earth. Lala initially runs away from home, citing she does not wish to get married to any of her arranged suitors. She enjoys making all manners of inventions (usually designed as pranks) and uses them frequently, often prompting some disastrous effect or another. Upon arriving on Earth, Lala pretends to love Rito to keep herself from getting married, but does exactly what she did not intend, after she misinterprets what Rito shouts in self-defence. Throughout the series, she always tries to get Rito to love her more, and barely shows any form of negativity towards him; she even states it is impossible for her to hate him.